Yeast Deformation: Initial Report

Yeast Deformation: Initial Report

by

Clifford E Carnicom
Sep 22 2017

 

A yeast culture that has been subjected to an isolated protein is under study. This protein is described in greater detail in the paper entitled, Morgellons: Unique Protein Isolated and Characterized (Aug 2017). This protein is derived from the microorganism tentatively identified as a ‘cross-domain bacteria’ (CDB) as described more extensively on this site.

The purpose of the project is to explore the impact of the protein upon more rudimentary life forms; in this case, a fungus. The protein concentration solution applied to the yeast culture is 0.5% by weight. Control solutions with the use of water and sucrose alone are conducted in parallel for comparison.

The result of this experiment, at this early stage, is that a cellular deformation or alteration of significant proportion has taken place. This suggests that the early growth of this particular fungus is modified in a significant fashion with the inclusion of this protein in the nutrient medium. The act of mutation must be considered as a distinct possibility in this case.

The change occurs primarily upon a surface layer that forms within the culture; this same layer does not develop within the control culture of water and sucrose alone. The act of change is a division process that appears to frequently “join” cells into doublets or triplets, as opposed to a full bud spherical division as expected.

 

control_01.jpg control_02.jpg

Control growth yeast cells in sucrose and water solution. 72 hour growth period. Cells are generally circular in shape and symmetric. Normal budding and division reproduction process. The appearance of the culture is normal and stable. Magnification approx. 5000x.

 

protein_01.jpg protein_02.jpg
Yeast culture subjected to water, sucrose, and specific protein solution. The isolation of the protein is described further within the research of this site. Concentration of the protein is 0.5% by weight. 72 hour growth period. Unusual growth alterations are evident. Doublet and triplet cell formation appears to be common within the population. Magnification approx. 5000x.

 

The growth process of the yeast culture will continue to be monitored.

Clifford E Carnicom
Sep 22 2017

Born Clifford Bruce Stewart
Jan 19 1953

MORGELLONS AND RECENT FINDINGS

MORGELLONS  AND RECENT FINDINGS:

PART I : MORGELLONS : A REVERSAL STRATEGY

PART II : PROTEINACEOUS FORM IDENTIFIED

PART III : DIMORPHISM, SYMBIOSIS OR DESIGN

PART IV: MAGNETIC PROPERTIES OF THE GROWTH FORMS

PART V : DNA EXTRACTION

PART VI: THE SERBIAN SAMPLE

PART VII : COLUMN CHROMATOGRAPY

PART VIII : CONFERENCE VIDEO EDITING PROJECT

PART IX : CULTURE GROWTH RATE IMPROVED

PART X : ELECTROPHORESIS PROCESS BEGINSPART

XI : ANOTHER POSITIVE TEST METHOD FOR IRON (Fe+3) IN THE CULTURE

IN PROGRESS
Estimated Completion Date : Can Not Be Estimated At This Time

Clifford E Carnicom
Jan 2012

Note: I am not offering any medical advice or diagnosis with the presentation of this information. I am acting solely as an independent researcher providing the results of extended observation and analysis of unusual biological conditions that are evident.  Each individual must work with their own health professional to establish any appropriate course of action and any health related comments in this paper are solely for informational purposes and they are from my own perspective.

 

PART I : MORGELLONS : A REVERSAL STRATEGY (Dec. 18, 2011)

A viable and tangible strategy to disrupt the growth process of the Morgellons condition, as it exists within the culture form that has been developed, has been established.  This strategy involves the breakdown of certain chemical bonds within an identified proteinaceous complex in a manner that is not harmful to the human body.  The reduction strategy also includes the release of iron that is held within the proteinacous complex in a chelated form.  This strategy has been established with confidence and a repetition of results.  The current work will be applied next directly to oral human samples.  Much time, energy and resources will be required to further investigate, verify and apply this strategy. The preliminary results and the theories are promising at this stage.

biuret iron

To be continued

protein graph

To be continued

PART II: PROTEINACOUS FORM IDENTIFIED

A note to the staff of the Institute tonight (Dec. 2, 2011); this will give some idea as to some of the work in progress…


The existence of a protein within the culture growths has now been established with confidence tonight. I had to do work to eliminate questions of potential contaminants that might have distorted the results. It is also a process of much patience with chromatography, literally drip by drip over many days for each test that is set up. It has taken about 1 1/2 to 2 months to get to this point.

Existence of a protein is eventually of equal importance as that of the iron work. We now have iron and the protein as two primary and identified constituents. This work will raise more questions that it answers, but we need to live with this for now until future means and equipment and methods work their way in. One more reliable way of putting a stop to this fellow is to truly understand the biochemistry and the life cycle of growth; there is then a better chance of interfering with that cycle in a known manner.

The existence of a protein means there is DNA behind it. As you can imagine, the work has actually just begun if we can get these means. Next questions would be what type of protein, what is the function of the protein(s), sequencing of the proteins, etc. Right along with it would be the isolation of DNA, electrophoresis work, etc.  An infra-red spectrophotometer would be a very useful piece of equipment for us on an ongoing basis – we are having to work very hard to get certain results that would be more apparent with the right equipment.

I may put this comment on the paper to get the process started, otherwise I have so many to write I will never get to any of them at the current rate…

Clifford

 A positive Biuret protein test result

A positive Biuret protein test result using a separation of elute from the chromatography column. The sample material is based upon a culture from oral filaments.  The original extraction from the chromatography column is to the left; the positive Biuret result for the existence of a protein is shown on the right with the purple color.  Successful separation on the column has been achieved using various combinations of solvents in combination with a stationary phase

A positive Biuret test result using whey

A positive Biuret test result using whey (lactoferrin) protein for control purposes.  A positive test results in the purplish color shown above.  The Biuret test depends on a copper complex that forms between the protein (peptide bonds) and copper sulfate and an alkaline solution, such as sodium hydroxide.

PART III: DIMORPHISM, SYMBIOSIS OR DESIGN

The morphology, metabolism and life cycle of the “Morgellons” organism, as defined by this researcher, is increasingly being understood.  There are now three scenarios that can be provided that encompass the majority of the understanding that has been achieved.  

The first of these examines a similarity of form, at least in part, to a dimorphic fungal-like organism.  

The second considers the joint existence of bacterial-like and fungal-like organisms in a symbiotic relationship.  

The last raises the spectre of a genetically created or designed organism.  

Each of these scenarios has certain strengths, weaknesses and probabilities of occurrence.  There can also be a degree of overlap between these alternative interpretations.  This paper will discuss what has been discovered, within these three scenarios,  that helps us to potentially define the nature of this unusual organism.

morphology 1

morphology 2

morphology 3

morphology 4

morphology 5

morphology 6

morphology 7

morphology 8

morphology 9

morphology 10

morphology 11

morphology 12

morphology 13

morphology 12

morphology 13

PART IV: MAGNETIC (ELECTROMAGNETIC) PROPERTIES OF THE GROWTH FORMS:

The magnetic (and consequently, the electromagnetic) properties of the primary Morgellons growth form are now proven in a direct fashion.  The video segments below show the response of both the culture derived form and the oral sample to a strong magnetic field.  These demonstrations will call into consideration each of the papers written on the subject of electromagnetics by this researcher.  One such topic will be the extended research that has been done that reveals the ambient presence of unaccounted Extremely Low Frequency (ELF) energy over a testing period of several years.  The human electromagnetic system operates primarily within the ELF portion of the electromagnetic spectrum.  The sensitivity and response of the Morgellons growth form to the electromagnetic spectrum is another of the many primary fields of research that requires funding, resources and skilled personnel to complete.  The identified presence of iron and ferromagnetic compounds within the growth forms establishes the basis of this future research, along with the direct demonstration of the magnetic response shown below:


To be continued.


PART V: DNA EXTRACTION

dna 1 dna 2 dna 3

To be continued.


PART VI: THE SERBIAN SAMPLE

To be continued.

serbia 1 serbia 2
serbia 3
serbia 4 serbia 5
serbia 6 serbia 7
serbia 8

PART VII: COLUMN CHROMATOGRAPHY

To be continued.

column 1

column 2

 

PART VIII : CONFERENCE VIDEO EDITING PROJECT

 

To be continued.

PART IX : CULTURE GROWTH RATE IMPROVED

To be continued.

X : ELECTROPHORESIS PROCESS BEGINS

ELECTROPHORESIS 1

Starch Gel Electrophoresis Applied to Proteinacous Samples : Initial Tests Underway

ELECTROPHORESIS 2

ELECTROPHORESIS 3

Starch Gel Electrophoresis : Trial Runs of Test Dyes and Blood Sample.   Left photograph shows methylene blue dye migration towards the negative terminal. Arrows on right photograph depict origins of placement.  Blood sample shows both positive and negative charged protein component separation at lower portion of right photograph.  Eosin test case on upper left of right photograph; migration toward positive terminal  Methods remain under development; no successful separation of presumed culture based proteinacous component at this time.

To be continued.

XI :ANOTHER POSITIVE TEST METHOD FOR FERRIC IRON (Fe3+) IN THE CULTURE

Another test method has been developed to detect and establish the presence of iron in the Fe3+ state within the culture growth that is based upon the oral samples.  The test is positive.  The further significance of this test is that it has been applied directly to the proteinaceous complex that has been extracted from the culture with the use of column chromatography.  This further substantiates the case that the proteinaceous complex itself contains iron in the ferric state and that this iron is bound to certain amino acids that are under examination as candidates.   It will be possible to determine the concentration of the iron within the proteinaceous complex through spectrometry.  The test is based upon the use of ammonium thioglycolate.

Clifford E Carnicom
(born Clifford Bruce Stewart Jan 19 1953)

DNA CULTURE RESULTS

 DNA CULTURE RESULTS
Clifford E Carnicom
Dec 28 2009

I am not offering any medical advice or diagnosis with the presentation of this information.  I am acting solely as an independent researcher providing the results of extended observation and analysis of unusual biological conditions that are evident.

A method of extracting DNA samples from living forms has been established.  The protocol being followed is that from the Genetic Services Learning Center at the University of Utah1.  The methods have been applied successfully to human and fruit samples.  Equipment to examine the internal structure of the DNA samples is not available at this time; visual microscopy techniques at relatively high magnification (~10,000x) are available.

The initial finding is that the Chlamydia-like organism under extensive study with respect to the so-called “Morgellons” condition occurs in relatively large numbers within the human DNA samples that have been studied and that it can be readily identified with sufficient magnification.  This further confirms the supposition that this particular organism appears to broadly distributed within human physiology, and that its existence should not be restricted to blood sample examination.  Thus far, this particular organism has been found within dental samples, saliva samples, urinary samples, red blood cells (erythrocytes) and anomalous skin filaments.  The particular DNA sample examined here is developed from human saliva.

INITIAL RESULTS :

human dna

human dna 2

Human DNA sample extracted from saliva.  .
Magnification approx 300x.

Human DNA sample extracted from saliva.  .
Magnification approx 300x.

organism identified

additional representative cluster

The Chlamydia-like organism identified within the human DNA sample.  .  This photograph is taken from the original sample prior to any cultured result.  The size of the individual organism, as reported previously, is approximately 0.5 to 0.8 microns.  The limit of conventional light microscopy is. approximately 1000-2000x.  Magnification approx.10,000x.

An additional representative cluster of the Chlamydia-like organisms identified within the human DNA sample.  .  This photograph is taken from original sample prior to any cultured result.  The size of the individual organism, as reported previously, is approximately 0.5 to 0.8 microns.
Magnification approx.  .  10,000x.

Secondly, the Chlamydia-like organism has been successfully cultured from its origin in the human DNA sample.  The culture medium is again red wine.  Most biochemical reactions take place within a specific pH range, and the chemistry of wine will become increasingly important in the understanding of why this particular medium is repeatedly favorable toward a multitude of culture developments under way.  The chemistry of wine is relatively complex and eventually the various components that are favorable toward growth will require isolation and identification.  It is presumed that the pH of red wine (acidic) will be one important factor to be identified within this future analysis as it takes place; a first hypothesis may be that this pathogenic form favors an acidic environment within the body.  It is also reasonable to suggest the hypothesis that a shift toward increased alkalinity within the growth medium may eventually serve as an inhibiting growth factor.  The current culture under analysis is approximately 7-8 weeks of age.

CULTURE RESULTS :

red wine medium

red wine medium #2

A culture of the DNA sample in a red wine medium.  .  The earlier stages of the identified pathogenic cycle are contained within this level of growth.  This includes extensive development of the Chlamydia-like organism, the pleomorphic structures (tentative candidate is Mycoplasma-like) and the eventual encasing filament structure (red arrows).  The erythrocytic form is not identified within this culture.  Age of culture is approximately 7 to 8 weeks.
Approx.  magnification 300x.

An additional view of the culture of the DNA sample in a red wine medium.  Arrows point to the early development of the filament stage of the growth cycle.  The background growth is composed primarily of the Chlamydia-like organism.  The age of the culture is approximately 7 to 8 weeks.
Approx.  magnification 300x.

developed form

developed form #2

A focus on the Chlamdia-like organism that has developed from the culture of the human DNA sample (red circle encloses four individual structures).  Magnification level approx.  10,000x.

Another.  example of the Chlamdia-like organisms that have developed from the culture of the human DNA sample (red circle).  In addition, the pleomorphic and early stages of the filament form (blue arrows).  are also contained within this photograph.  Magnification level approx.  10,000x.

Third, the DNA sourced culture that has been developed demonstrates identical growth to that which develops from the dental filament cultures.  

In addition, as reported earlier, an environmental source for an identical growth cycle has been established; this is the unusual airborne filament sample that has been extensively reported on over the years.  The identification of the nature of this filament sample has been refused by the U.S.  Environmental Protection Agency (EPA).  Please refer to the paper entitled “Morgellons : An Environmental Source” for additional documentation on this recent development.  

Lastly, It is now to be reported that the growth from the DNA culture as shown in this report is identical (to the magnification level that is available) to that of the airborne environmental sample culture described in the earlier report .

The level of congruence between environmental sampling, biological sampling, and culture developments is sufficient to merit extensive and detailed study and discussion with respect to the Morgellons condition.  Additional preliminary studies also indicate that these examinations should be extended beyond consideration of the human organism.

Reference:

1.  .  How to Extract DNA from Anything Living, University of Utah, Genetic Sciences Learning Center, http://learn.genetics.utah.edu/content/labs/extraction/howto/

THE MONITORS OF JGI

THE MONITORS OF JGI
Clifford E Carnicom
Mar 17 2003

The United States Department of Energy (DOE) Joint Genome Institute (JGI) (associated with Lawrence Berkeley  National Laboratory) has recently demonstrated a heightened interest in the research materials available on www.carnicom.com.  This interest appears to correlate with a recent presentation on the topic of atmospheric tests conducted for the presence of mold.  That report may be found at the page entitled : An Abundance of Mold.

The mission of the Joint Genome Institute (JGI) is stated as follows: 

Mission Statement

* To develop and exploit new sequencing and other high-throughput, genome-scale and computational technologies as a means for discovering and characterizing the basic principles and relationships underlying the organization, function, and evolution of living systems.

* To leverage the unique capabilities of the Department of Energy National Laboratory system to achieve these goals, and to use the resulting understanding to address key DOE [Ed : Department of Energy] missions related to energy, the environment, and human susceptibilities.

An information page on microbial genomic programs from this institute can be found at:

http://www.jgi.doe.gov/JGI_microbial/html/